Proliferation of the released daughter cells was monitored at a wavelength of 578 nm online by a microplate reader (VersaMax; Molecular Devices, Sunnyvale, Calif. The Molecular Devices VersaMax Microplate Reader is a high-performance laboratory instrument designed for fast and accurate measurements of absorbance, fluorescence, and luminescence from 6 to 384-well microplates. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Conventions Used in this Manual. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. used. g. The plates were incubated at RT for an additional 25 min to stabilize the luminescence signals followed by the measurement of luminescence using a microplate reader (VersaMax, Molecular Devices, CA, USA) (n = 6). 0 OD. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. reader (VersaMax, Molecular devices, San Jose, CA, USA). Oil red O staining. Unit price / per . SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PMThis version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). The cytokine concentration was calculated from the standard curve using seven-parameter curve fitting software (SOFTmaxPro 5. Select Add or Remove Programs system settings. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The viability of the treated group was expressed as a percentage of non-treated control group, which was assumed to be 100%. After freeze-drying, the. We would like to show you a description here but the site won’t allow us. Used. Supplier: Molecular Devices. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. reader (VersaMax, Molecular devices, San Jose, CA, USA). Glossary of Terms. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. This technical note is designed to provide information . The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. 파장 범위(nm) : 340–850. Our mission: find your user guide or user's manual as quickly as possible. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. SpectraMax®, VersaMax, VMax. Using a Spectradrop Micro-Volume Microplate (VersaMax, Molecular Devices, CA, USA), RNA was quantified, and 1 μg of total RNA was treated with DNase I (Thermo Scientific, Waltham, MA, USA) before. Cell viability was analyzed using a cell counting kit assay (Dojindo). The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Animals and preparation of tumor model. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. VERSAMAX. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. Preconfigured method protocols specifically written for the Pyrochrome Assay and other ACC assays are available for SoftMax Pro Software and take. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. This manual is subject to change without notice. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. g. The absorbance was detected at 517 nm wavelength using a microplate reader (VersaMax, Molecular devices, USA). ImmunohistochemistryAfter 1 h incubation at 37 °C, the color intensity was measured at 562 nm by a microplate reader (model VERSAmax; Molecular Devices, San Jose, CA, USA). FRAP solutions were prepared as described previously (Benzie and Strain, 1996, Benzie and Strain, 1999). We would like to show you a description here but the site won’t allow us. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. to 11. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Absorbance plate reader (e. Bovine serum albumin was used as a reference. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. ® US HEADQUARTERS. Technical Specifications. FAST AND ACCURATE METERTECH Microplate Reader $3,650. 5 mM. , Spectramax Gemini; Molecular Devices) Tissue culture plates (24- and 96-well) Vacuum suction pumpRelated products. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 4 0112-0126B SafetyInformation InformationaboutthesafeuseoftheinstrumentfromMolecularDevices®includesan Get the most out of SoftMax Pro. Monday to Friday 8am - 5pm GMT. ¨ We would like to show you a description here but the site won’t allow us. After treatment, cells were collected, lysed with the lysis buffer. Finally, the optical density of the wells was determined at an excitation wavelength of 450 nm and a reference wavelength of 540 nm on a VersaMax (Molecular Devices) microplate reader. Enzyme activity was. Measurement of biofilm formation inhibitory activity. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. 3, Molecular Devices). Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. , Sunnyvale, CA). The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. (JC-1) (Molecular Probes), respectively. StakMax Microplate Handling Stacker. The. . 9000-0756. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. S. ChE activity was normalized by protein concentration as determined by a Coomassie. 4. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. It features a modular design that allows users to add or change detection cartridges according to their needs. 8 in. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. Cell survival rate of each sample was calculated as a percentage relative to cells incubated in D-MEM. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. The calibration curve was fitted to a sigmoid by the four-parameter method in the SoftMax Pro software (Molecular Devices), and allowed the calculation of the concentration of native Antigen 18A. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. Lipid accumulation was assessed by ORO staining. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. 2 mM PLP and 5. Louis, USA) was used to. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA were put in each well. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. E. It features a modular design that allows users to add or change detection cartridges according to their needs. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). Fluorescence-activated cell-sorting analysis For analysis of DC surface molecules, 5×10 5 DCs were treated with soluble OVA (20 μg/mL), PLGA NPs (20 μg/mL OVA), PEI-coated PLGA NPs (20 μg/mL OVA), or 1 μg/mL. 0A 2 Lines Fused, unplug before ser. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. The water extract afforded the greatest yield, and total phenolic and tannin. S. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). Total. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. StakMax Microplate Handling Stacker. Cell viability was assessed using the WST-1 assay. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Finally, the absorbance at 450 nm was detected by an ELISA reader, VERSAmax (Molecular Devices, San Jose, CA, USA). Then, the cultured cells were treated with different. 5) containing 0. Table of Contents. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. $199. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. 0200-6117. S. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. A. The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. Intracellular triglyceride assay. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Use Windows Search to search for “Add or Remove”. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. 0, 0. Find regional support. Molecular Devices Corporation. The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). 0 out of 5 $ 2,999. Select up to two wavelengths. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. To update the driver. We would like to show you a description here but the site won’t allow us. No accessories are needed and SoftMax® Pro Software. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 747. Reboot the computer. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,Get the most out of SoftMax Pro. SoftMax® Pro 7. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. If the computer is connected to the Internet: Select Search Automatically for. 00. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. SoftMax Pro. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. High-Throughput Screening. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Furthermore, VersaMax is reliable, which means more uptime and lower long-term cost of. 0 out of 5 $ 23,995 $ 22,495. This document is provided to customers who have purchased Molecular Devices, Inc. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. 340‚ 850 nm. The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). Am J Respir Crit Care Med 1998; 157: 351–370. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). 0%, 0-2. The sections were stained with hematoxylin and eosin (H&E) and. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). HT22 cells are derived from hippocampal neuronal HT4 cells. used. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. Spectranet customer portalSpectraMax and VersaMax . 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Supplier: Molecular Devices. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Software (optional), such as Softmax Pro (Molecular Devices). In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. This manual does not represent a commitment on the part of Molecular Devices Corporation. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. While the cell covers about. Taking into account the price differences between each seller and each country. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Add to Cart. The concentrations of anti-RSV G protein IgG antibodies in the samples were. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. 1 N NaOH (50 μl) followed by absorbance measurement at 405 nm with a plate reader (VersaMax, Molecular Devices, Sunnyvale, CA). Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including: vaccines. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. MOLECULAR DEVICES VMAX KINETIC MICROPLATE READER. 7. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. 8). The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. View the supported Window operating systems for the different versions of the SoftMax Pro software. The reaction in the wells was stopped by the addition of 50 μl of 1 M sulfuric acid, which turned the wells yellow. The absorbance was assumed to be 100% for the control group. Absorbance was read on a spectrophotometer (VersaMax, Molecular Devices) with SoftMax Pro GxP v5 software. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Fabrication of Microfiber-Based Strain SensorsWe would like to show you a description here but the site won’t allow us. BMG Labtech - THERMOstar. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). We would like to show you a description here but the site won’t allow us. HepG2 cells were seeded into 6-well plates. Table of Contents. 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. VersaMax™ Tunable Microplate Reader, Molecular Devices. 68 $. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Resveratrol (1 µ M) was expected to produce a sufficient effect on ER-stress and ROS production and was applied for subsequent analysis; a dose-dependent effect was also investigated by using 1 and. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). 3 Methods 1. Windows 10 – 2004/20H2/21H1/21H2. TECHNICAL NOTE. 4. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. Find regional support. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. 00. Sale Sold out. Add to Cart. We would like to show you a description here but the site won’t allow us. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Measurement of changes in BDNF production and release was done byMicroplate Readers. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. All animal experiments were performed in compliance with the relevant laws and. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). spectrophotometer (VERSAmax, Molecular Devices). The. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . Learn how to use SoftMax. The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. , VersaMax; Molecular Devices) Incubator for cell culture. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. Nude mice were obtained from Orient Bio Inc. The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. 747. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. That's why our team is at your disposal the majority of time. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. TECHNICAL NOTE. Regular price $ 0. The emodin thermoreversible PG was. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. Then, the absorbance at a wavelength of 450 nm was measured using an absorption microplate reader (VersaMax, Molecular Devices, USA) to evaluate the cell survival rate. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. Narrow bandwidths deliver increased accuracy and enhanced peak. Table 1. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0 out of 5 $ 2,999-20%. com ©2010 molecular devices, inc. Finally, the absorbance was determined at 490 nm on a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA). These models can no longer be repaired. 2. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. Printed in U. 0200-6117. Molecular Devices, LLC. It also integrates with the. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. Wavelength Bandwidth °¤ 2. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The cellular viability. VERSAMAX - VersaMax Tunable Microplate Reader - VersaMax Tunable Microplate Reader, Molecular Devices - Each Brand: Molecular Devices $20,893. 23,225, Thermo Fisher Scientific). Next, 100 μL of a reagent was added to an equal volume of cell culture medium present in each well. Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 0 nm full width half maximum. 4. StakMax. The limit of detection for each assay was 156. ® SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All. To update the driver. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. Reverse transcription-quantitative polymerase chain reaction To assess phospholipase C gamma 1 (PLCG1) and phospholi-pase C gamma 2 (PLCG2) mRNA levels in each. [Google. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). 3860 N First Street San Jose,CA 95134 USA +1 408. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. S. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Select Start>Control panel>Device Manager. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. We would like to show you a description here but the site won’t allow us. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Click SoftMax Pro Software User Guide. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pm SpectraMax and VersaMax . Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. 10. # SMP54-GXP- 10UL) Pyrochrome Assay. After incubation for 1 h, the medium was transferred to 96-well plates (SPL Life Sciences) in triplication and absorbance was measured by using the ELISA microplate reader (VersaMax, Molecular Devices, USA) at 490 nm. Published Dec 16, 2020 | Updated May 19, 2023. The absorbance of the treated and untreated samples at 450 nm was then measured on a multi-well microplate reader (VersaMax, Molecular devices, San Jose, CA, USA). Absolute values were attained with a standard curve composed of type I bovine skin collagen solution (0. 0 software (GraphPad). Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. Designed to provide the simplicity, flexibility and power required for advanced data analysis. (Molecular Research Center, USA) according to the manufacturer’s. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. All experiments were repeated three times. S. Table of Contents. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The absorbance of reaction mixtures was read at 475 nm using a microplate reader (VERSAMax, Molecular Devices) to determine the quantity of dopachrome produced. We would like to show you a description here but the site won’t allow us. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. The growth inhibitory rate (GIR) of treated cells was calculated by Equation 1. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. The. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Multichannel optics. We would like to show you a description here but the site won’t allow us. SpectraTest® Validation Package. Molecular Devices VersaMax Microplate Reader Molecular Devices VersaMax Microplate Reader Specifications. 1700 +1 800. 9000-0756. S. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader.